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Research Article

Academia Journal of Agricultural Research 3(9): 184-188, September 2015
DOI: 10.15413/ajar.2015.0145
ISSN: 2315-7739
2015 Academia Publishing

 

Abstract

Micropropagation of four potato cultivars in vitro

 


Accepted 7th August, 2015

 

Fawzia Abo-Sria Ebad1, Marwa EL-Sebai Abd EL-sadek1 and EL-Kazzaz A.A2*

1Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Nasr City, Cairo, Egypt.

2Plant Biotechnology Department, National Research Centre, 12622, Dokki, Giza, Cairo, Egypt.

*Corresponding author e-mail: profelkazzaz3@yahoo.com

In vitro micropropagation is an alternative to conventional (vegetative) propagation of potatoes whereas aseptically meristem cultures were used which gave pathogen free plants. However, three concentrations of disinfectant bleach (Clorox) 15, 20, 25% with two exposure time 15 and 20 min were used for disinfecting the isolated potato sprouts from four potato genotypes named Lady Rosetta, Jaerla, Cara and Hermis. It was found that, as simplest disinfection protocol, concentration 20% Clorox was the suitable one at 20 min of exposure time giving high percentages of survived individuals with low percentage of dead and contaminated individuals. The sterilized sprouts were cut to isolate apical meristems which were then cultured on shoot induction medium containing solidified MS medium with vitamins and free of exogenous plant growth regulators and incubated in a growth chamber at optimized culture conditions in room culture. The initiated shootlets from the aseptic meristem cultures were cut to nodal cuttings which were culture on the previous MS medium for mass propagation of potato plantlets in vitro. The results cleared that MS medium with vitamins and solidified by agar without exogenous plant growth regulators can be used for mass propagation of free-pathogen true to type of potato genotype in vitro under the optimized culture conditions.

 

Key words: Plant tissue culture, true to type, free-pathogen plants, meristem culture.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

Cite this article as:

Ebad FA, EL-sadek MEA, EL-Kazzaz AA (2015). Micropropagation of four potato cultivars in vitro. Acad. J. Agric. Res. 3(8): 184-188.

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