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Research Article

Academia Journal of Biotechnology 4(11): 372-383, November 2016
DOI: 10.15413/ajb.2016.0121
ISSN: 2315-7747
2016 Academia Publishing

Abstract


Production, Optimization, Partial Purification and Immobilization of protease from Newly Isolated Bacterial Strain T
3
 

Accepted 18th August, 2016

Mina Ilyas

Department of Microbiology, The University of Lahore, Pakistan
Email; mina_ilyas@hotmail.com, Cell. +923224509185

 

The research was conducted for protease production and its usage after immobilization. Enzyme production was optimized using submerged fermentation technique for different ranges of pH and different concentrations of different carbon and nitrogen sources. Fermentation media was incubated for 48 h at 37C temperature with agitation speed of 200 rpm. Protease production (6.4 U/ml/min) from bacterial strain T3 was optimized at 2.0% soybean meal, 1.5% glucose and 8 pH. Protease was partially purified with 70% ammonium sulphate and immobilized on different supports by physical adsorption and their activities checked. When partially purified protease was immobilized on Amberlite (XAD 761) then increase of protease production was 208.33% (25 U/ml/g support) for strain T3. When partially purified protease was immobilized on Duolite (A568), the increase of protease production was 225% (27 U/ml/g support) for strain T3 and when partially purified protease was immobilized on Lewatit (VPOC, 1600), the increase of protease production was 541.66% (65 U/ml/g support) for strain T3. When partially purified protease was immobilized on Pentynyl Dextran (NT4L360), the increase of protease production was 3483.33% (418 U/ml/g support) for strain T3. This immobilized protease with 35-fold increase in its activity may be used in food industry, pharmaceutical industry, bioremediation, detergent industry and textile industry etc.

Key words: Protease production, partially purified and immobilized protease, isolated bacterial strain T
3.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:

Ilyas M (2016). Production, Optimization, Partial Purification and Immobilization of protease from Newly Isolated Bacterial Strain T3. Acad. J. Biotechnol. 4(11): 372-383.

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