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Research Article

Academia Journal of Biotechnology 7(7): 128-132, July 2019
DOI: 10.15413/ajb.2019.0109
ISSN: 2315-7747
2019 Academia Publishing

Abstract


Evaluation of days-dependent chloramphenicol dosage on rat liver microsomal lipid peroxidation and catalase activity
 

Accepted 15th March, 2019
 
Ukoh, Sandra Nonyerem Bunmi* and Adesuyi, Adeola Alex

Cell Biology and Genetics Department, University of Lagos, Akoka, Lagos, Nigeria.


The cellular functions associated with oxidative metabolism can be altered by drugs and chemical agents, thereby stimulating ROS production. This research study is focused on the in vivo study of the inhibitory effect of chloramphenicol on hepatic microsomal enzyme system and the effect on lipid peroxidation using Thiobarbituric Acid (TBA) reactive as index of peroxidation damage. The rats were randomly divided into three (3) groups with group 1 serving as the control receiving saline water, while group 2 received chloramphenicol at a dose of 28.6 mg/kg body weight/day for 5 days and group 3 for 7 days. Liver protein content, lipid hydroperoxides and catalase activity were all determined. TBARS concentration significantly increased statistically with time of exposure. After 5 days of treatment, the level of TBARS increased by 103.56% with exogenous oxidant and 141.54% without oxidant, as compared to the respective control. On day 7, TBARS level in the liver was approximately 90.75% higher than in the control group with oxidant and 117.03% higher than the control without oxidant. The antibiotic elicited significant increase in rat liver lipid peroxidation compared to control. There were decreases in microsomal protein content of the liver in the drug-treated rats when compared with the control rats. It also showed that chloramphenicol treatment affects cytosolic catalase activities. It decreases catalase levels by 15.75% in 5 days dosage treatment and 17.81% in 7 days dosage treatment, respectively. There were significant difference (P<0.05) in the treated and control groups irrespective of the days of dosage. The catalase level in hepatocyte in 5 and 7 days group was significant at p<0.01. In conclusion, it was evident that treatment of rats for 5 and 7 days with the therapeutic doses of chloramphenicol altered the antioxidant system and the intensity of effects was concentration/dosage days dependent, which resulted in membrane lipid peroxidation, protein damage and inhibition of microsomal catalase due to increased generation of free radicals, reactive oxygen species (ROS) and reactive nitrogen species (RNS).

Key words: Chloramphenicol, rat liver, lipid peroxidation, catalase activity, antioxidant.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Ukoh SNB, Adesuyi AA (2019). Evaluation of days-dependent chloramphenicol dosage on rat liver microsomal lipid peroxidation and catalase activity. Acad. J. Biotechnol. 7(7): 128-132.

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