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Research Article

Academia Journal of Biotechnology 6(6): 101-106, June 2019
DOI: 10.15413/ajb.2019.0110
ISSN: 2315-7747
2019 Academia Publishing

Abstract


Studying the possible biotransformation of Antihistaminc Loratadine using Phaseolus vulgaris L. cell suspension culture
 

Accepted 27th June, 2019
 
Jailan E. Elhalawani1*, Ghada Farouk1, Haidy Gad2, Mohammed Ashour2 and Mohammed M. Al-Azizi2

1Plant Tissue Culture Laboratory, National Organization for Drug Control and Research, Giza, Egypt.
2Pharmacognosy and Phytochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.



The aim of this research was to perform biotransformation for Loratadine drug to either Desloratadine or denovo secondary metabolites using cell suspension cultures of Phaseolus vulgaris, of the family Fabaceae. A solid medium was developed for the cultivation of Phaseolus vulgaris L. seeds and establishment of callifrom hypocotyls. The inoculation of the cell suspension cultures and biotransformation of Loratadine was monitored using the produced calli and TLC and HPLC techniques. Seeds of P. vulgaris L. were germinated on MS solid medium and the hypocotyls excised and thereafter cultured on MS medium supplemented with BA (0.5 mg/L) and 2,4-D (5 mg/L). The resulted calli were used to establish different sets of cell suspension cultures in variable amounts (2, 4 and 8/150 ml), respectively. Loratadine was added to each set with different concentrations (5, 10 and 15 mg/150 ml) and after 10 days of incubation on the shaker the samples were withdrawn for 4 consecutive days (11th to 14th day). A callus line of P. vulgaris was maintained for four years without change in growth rate. The cells reached their maximum bioavailability on the 23rd day (around 4 weeks). On adding Loratadine with variable concentrations (5, 10 and 15 mg/ 150 ml) to callus cells (2, 4 and 8 g/150 ml), some peaks evoked as traced by HPLC after 10 days of incubation but not the same results were obtained in all cases. Desloratadine peak was evoked in nearly all cases but with trace amounts together with other secondary new metabolites formed. This would be the first report of a biotransformation trial using P. vulgaris cell cultures which gave highly positive results. This would be the first report on P. vulgaris to be used in biotransformation of Loratadine.

Key words: Phaseolus vulgaris, Loratadine, Desloratadine, cell culture, biotransformation.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Elhalawani JE, Farouk G, Gad H, Ashour M, Al-Azizi MM (2019). Studying the possible biotransformation of Antihistaminc Loratadine using Phaseolus vulgaris L. cell suspension culture. Acad. J. Biotechnol. 7(6): 101-106.

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