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Research Article

Academia Journal of Biotechnology 7(6): 107-115, June 2019
DOI: 10.15413/ajb.2018.0143
ISSN: 2315-7747
2019 Academia Publishing


Phenotyping using pilodyn as a tool, selective genotyping and preliminary genetic diversity estimation for wood basic density in Melia dubia

Accepted 30th May, 2019
Geeta Joshi, Shakti Singh Chauhan, Arunkumar, A. N, Ajay, T. L, Sreedevi, C. N, Aparna Rajan, Annapurna Dhavala*

Institute of Wood Science and Technology, PO 18th Cross, Malleswaram, Bengaluru, Karnataka, India – 560 003

Melia dubia Cav. (Meliaceae), a native species to India, is rapidly emerging as a commercially important tree species due to its fast growth and multiple uses. The wood has potential for plywood, pulping and high value solid wood products. Wood basic density is one of the favoured traits for selection as it influences many of mechanical and anatomical properties. Pilodyn Penetration (PP) which is an indirect measure of wood basic density was measured in a plantation (n=513) and it ranged from 14 to 25 mm in trees with ≥ 37.5 cm Girth at Breast Height (230 trees). An attempt was made to select phenotypes for wood basic density based on the extreme values of PP. Among the 230 trees, available trees for low PP (14 to 17 mm) were 16 (6.95%) and high PP (23 to 25 mm) were 19 (8.26%). These 35 phenotypes were used for selective genotyping and estimating of the genetic diversity. For genetic diversity estimation, bulks of low and high PP were screened with 63 markers and 12 markers showing traits related polymorphism were selected. Diversity estimates like number of observed alleles, heterozygosity, specific alleles and distinct alleles varied between two groups. Distinct alleles were observed in MSSR 2 for high basic density and MSSR 51 and MSSR 54 for low basic density. Cluster analysis resulted in three major clusters of which 51% of genotypes were distinctly segregated into two clusters having either low or high basic density. The study reconfirms that pilodyn can effectively be used for indirect estimation of basic density and these phenotypes can be used for selective genotyping for development of trait specific markers. Identifying more genotypes and markers of above type may facilitate future marker assisted selection programme for wood basic density in M. dubia.

Key words: Pilodyn, M. dubia, selective genotyping, wood basic density.


This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Joshi G, Chauhan SS, Arunkumar AN, Ajay TL, Sreedevi CN, Rajan A, Dhavala A (2019). Phenotyping using pilodyn as a tool, selective genotyping and preliminary genetic diversity estimation for wood basic density in Melia dubia. Acad. J. Biotechnol. 7(6): 107-115.

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