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Research Article

Academia Journal of Biotechnology 7(5): 084-090, May 2019
DOI: 10.15413/ajb.2018.0144
ISSN: 2315-7747
2019 Academia Publishing

Abstract


Genotyping of Indian cattle for common genetic disorders: Deficiency of Uridine Monophosphate Synthase and Factor XI
 

Accepted 30th November, 2018
 
Gebremedhin Gebreselassie1*, Sachinandan De2, Ayan Mukherje2 and Moloya Gohain2

1Department of Animal and Range Science, College of Dry land Agriculture, Jigjiga University, Ethiopia.
2Animal Biotechnology Center, National Dairy Research Institute, Haryana, India.


Deficiency of Uridine Monophosphate Synthase and deficiency of Factor XI are autosomal recessive inherited genetic disorders which affect all kinds of farm animals. Functional and physiological defects arising from such inherited disorders have negative impact on health and productivity and also reduce the rate of genetic improvement in cattle. This study was aimed to standardize PCR based test and be acquainted with the incidence of mutant alleles caused by two genetic disorders in cattle. Blood samples were collected from 327 cattle breeds: Karan Friss, Holstein Friesian, Tharparker, Haryana, Jersey and Red Sindhi. Genomic DNA from these animals was extracted and its quality assessed prior to PCR amplification. Primers were designed for DUMPS and FXI genes. An allele specific PCR technique was applied to amplify and detect mutation on the specific DNA target sequences. The amplified PCR products were purified and sequenced to confirm the change. Allele-specific PCR-based screening methodology was developed for Deficiency of Uridine Monophosphate Synthase and Factor XI cattle genetic disorders. All the tested animals were free from the aforementioned genetic disorders. However, this will not provide us conclusively cattle herds maintained at the study area free from these disorders. Therefore, it is highly suggested that screening works with large sample size should continue.

Key words: Autosomal, genetic disorders, genotyping, PCR optimization.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Gebreselassie G, Sachinandan D, Mukherje A, Gohain M (2018). Genotyping of Indian cattle for common genetic disorders: Deficiency of Uridine Monophosphate Synthase and Factor XI. Acad. J. Biotechnol. 7(5): 084-090.

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