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Research Article

Academia Journal of Food Research 9(2): 016-021, August 2021
DOI: 10.15413/ajfr.2021.0102
ISSN 2315-7763
©2021 Academia Publishing



Alleviation of drought stress of tomato plant regenerated via tissue culture inoculated with Bacillus megaterium (ATCC® 14581™)


Accepted 20th September, 2021

Deodata V. Mtenga1,3 and Asha S. Ripanda2

1College of applied Sciences, Palestine Technical University, Palestine.
2Faculty of Agriculture and Veterinary Medicine of veterinary, Annajah National University, Palestine.
3Department of applied and molecular Biology, Palestine Technical Kadoorei- B.O7, Jaffa street, Fax: +970 9 2677922. Tulkarm, Palestine.


Drought is one of the major limiting factors affecting crop productivity worldwide. Middle East in general is affected by water scarcity that mainly affects crop production. Tomato is one of the major stable vegetables consumed daily by Palestinian. This crop is drought sensitive; therefore, searching for reliable methods that enhances drought tolerance is highly favorable by farmers. One of the promising methods is utilizing a natural root colonizing bacteria to alleviate drought stress. In this work, a study was conducted to explore the efficiency of bacteria Bacillus megaterium (ATCC® 14581™) inoculum in enhancing the growth and physiological parameters of tomato (Solanumlycopersicum, L.) Var. Azmir plant derived via plant tissue cultured and normal seed derived plant when subjected to two levels of drought regimes. Under both drought-stress and non-stressed conditions, this bacteria successfully enhanced growth and physiological parameters. No significant differences were seen in all tested parameters between tissue culture and seed- derived plants. In general, high inoculum concentration inversely affects growth and physiological parameters regardless of the source of plant (treatment 4). The averages of shoots fresh weight, dryweight and plant height increased by 46.4%, 21.7% and 24.4% respectively than non-inoculated plant, while chlorophyll a, photosynthetic rate, and stomata conductance and respiration rate also increased by 54 %, 49 %, 39 % and 64%, respectively than non-inoculate plant( treatment 2). Exposure of inoculated plant to 8-week drought-stress (75 and 50% of total water amount required) enhanced the averages of shoot fresh weight, average dryweight and plant height by 20 and 23%, 24% and 25%, 34%, 30% respectively. Chlorophyll a increased by 51.9% and 46%. The photosynthetic rate increased by 39 and 45 %, stomata conductance increased by 32 and 50% and transpiration rate increased by 37.8and 25 % respectively (treatment 2).

Key words: Tomato, Drought stress, Bacillus megaterium (ATCC® 14581™), endophyte, BAP, IAA.

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Sholi NJY, Abusafieh D, Al -Fares H (2021). Alleviation of drought stress of tomato plant regenerated via tissue culture inoculated with Bacillus megaterium (ATCC® 14581™). Acad. J. Food. 9(2): 016-021.

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