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Classifications

  1. Acad. J. Med. Plant.


ajmp

Research Paper

Academia Journal of Medicinal Plants 9(4): 040-047, April 2021
DOI: 10.15413/ajmp.2020.0145
ISSN: 2315-7720
2021 Academia Publishing

Abstract


Quantification of Diosgenin content in Dioscorea bulbifera linn germplasm from Uganda



Accepted 23rd December, 2020

 

Hilda Ikiriza1, Clement Olusoji Ajayi1, Hedmon Okella1, Abubaker Muwonge3, Mai Abdalla Ali Abdalla4 and Casim Umba Tolo1, 2

1Pharm- Biotechnology and Traditional Medicine Centre, Mbarara University of Science and Technology, P.O. Box 1410, Mbarara Uganda
2Department of Biology, Mbarara University of Science and Technology P. O. Box 1410 Mbarara, Uganda
3National Agricultural Research Organization (NARO), National Crops Resources Research Institute (NaCRRI), P.O. Box 7084 Kampala, Uganda
4Department of Biochemistry and Molecular Biology, Faculty of Science, Gezira University, P.O. Box 20 Wad Medani, Sudan.

*Corresponding author. E-mail: hikiriza@std.must.ac.ug.



 

Diosgenin is mainly found in wild Dioscorea sp. and is a well-known precursor of steroidal and contraceptive drugs. However, due to large market demand and increase in ecological damage, available wild Dioscorea spp. specifically Dioscorea bulbifera is gradually declining. This study therefore quantified the amounts of Diosgenin in D. bulbifera accessions in Uganda for conservation of high yielding germplasm. Mature aerial bulbs of D. bulbifera were collected from various regions of Uganda, that is; the Eastern (Mt Elgon forest reserve), Northern (Abur-buru forest reserve), Western (Kasyoha Kitomi forest reserve and Bugoma central forest reserve), Southwestern (Bwindi Impenetrable forest) and Central (Mabira central forest reserve). Samples were separately washed, shredded into small pieces and lyophilised. The freeze-dried samples were powdered mechanically and kept in polythene bags for extraction. The powdered samples were macerated with 70% ethanol, concentrated in vacuo to dryness. The extracts were reconstituted with water and defatted with hexane in a separating flask before they were partitioned with n-butanol. The butanol layers were concentrated and analyzed using High Performance Liquid Chromatography (HPLC) and also qualitative phytochemistry was done on the crude extracts. Results obtained from HPLC showed the highest concentration of diosgenin in Mabira forest reserve population with 0.996a mg/g, while Bwindi impenetrable forest population and Mt. Elgon populations had significantly lower concentration of 0.36e and 0.373e mg/g respectively at a retention time of 2.803 min. The method employed in current study confirmed a significant variation in the diosgenin concentration in Ugandan D. bulbifera populations except for germplasm from Bwindi and Elgon. The Diosgenin quantities were highest in Mabira central forest reserve with 0.996a mg/g implying the germplasm from Mabira would be ideal for conservation.



Key words: Dioscorea bulbifera, germplasm, Diosgenin, HPLC, phytochemicals, Uganda.




       

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Ikiriza H, Ajayi CL, Okella H, Muwonge A, Ali Abdalla MA, Tolo CU (2021). Quantification of Diosgenin content in Dioscorea bulbifera linn germplasm from Uganda. Acad. J. Med. Plants. 9(4): 040-047.

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