Grape’s antioxidants are associated with anti-inflammatory activity which is apparently related with chemo preventive actions. Since grapes come in various colours and varieties, it becomes essential to know which one is more beneficial and what kind of benefits it accounts for. Current research was carried out for evaluating in vivo antioxidant activities of green and black grape berries (Vitis vinifera L.) against DMBA/Croton oil induced oxidative stress. The effect of methanolic green and black grapes separately and in combination was evaluated to modulate oxidative stress in swiss albino mice through lipid peroxidase test (LPO), superoxide dismutase (SOD) method and reduced glutathione (GSH) estimation. Swiss albino mice were divided into five experimental groups according to their treatment procedures. According to the result, black grape methanolic extract was potent in preventing the damage caused due to oxidative stress followed by green + black grape methanolic extract and green grape methanolic extract as compared to control group.



Key words: Vitis vinifera L., chemoprevention, in vivo test, antioxidant activity, swiss albino mice, DMBA/Croton oil, oxidative stress, green grapes, black grapes, methanolic extract, LPO, SOD, GSH.