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Research Article

Academia Journal of Microbiology Research 4(3): 047-052, March 2016

DOI: 10.15413/ajmr.2016.0105
ISSN: 2315-7771
©2016 Academia Publishing


Abstract


A Novel Determination Method quantifying Serratia marcescens prodigiosin Concentrations Independent of Acidic and Alkali Conditions.
 

Accepted 3rd March, 2016

 

Yang Peizhou1,2*, Zhang Danfeng1 and Jiang Shaotong1,2


1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.

2Key Laboratory for Agriculture processing of Anhui Province, Institute of Agricultural Products Processing Technology, Hefei University of Technology, Hefei, Anhui 230009, China.


*Corresponding author e-mail: 1240818208@qq.com. Tel: 086-15155197790. Fax: 086-0551-62901507

 

 

Prodigiosin is a bioactive secondary metabolite produced by Serratia marcescens. Due to the difference of the acidic and alkali conditions, the absorption wavelength changed greatly using the absorbance value of 535 nm as the standard. It was difficult to accurately determine the prodigiosin concentration in vitro and in vivo. In this study, we proposed an accurate, reliable, and convenient method to determine the concentration of prodigiosin. Prodigiosin concentration was evaluated using the standard curve equation in accordance with the absorbance at a wavelength of 492 nm. For each standard curve equation, there remained steady linear correlations when prodigiosin was dissolved in methanol, ethanol, acetone, and butanol. Compared to the former method that used the absorbance level of 535 nm, the main advantage of this method is independent of pH value. The proposed method is conveniently used for general concentration measurements.

Key words: Prodigiosin, concentration, 492 nm, wavelength, Serratia marcescens, acidic and alkali conditions.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

Cite this article as:
Peizhou Y, Danfeng Z, Shaotong J (2016). A Novel Determination Method quantifying Serratia marcescens prodigiosin Concentrations Independent of Acidic and Alkali Conditions. Acad. J. Microbiol. Res. 4(3): 047-052.

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