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Research Article

Academia Journal of Microbiology Research 9(3): 055-068, August 2021
DOI: 10.15413/ajmr.2021.0112
ISSN 2315-7771
2021 Academia Publishing


Abstract


Detoxification of mycotoxin-aflatoxin B1 by soil bacterium  Pseudomonas toyotomiensis
 

 

Accepted 26th July, 2021


Manjunatha AS

Ramaiah College of Arts, Science and Commerce, Bengaluru-560054, India.

 

Aflatoxins bring great significant threats to food, feed industry and detrimental effects on humans including carcinogenic, mutagenic and teratogenic. The aim of this research was to explore efficiency of Pseudomonas sp. strain ISRH6 to degrade aflatoxin B1 concentration of (2 μg/mL) as a sole carbon source isolated from soil sample. The residual AFB1 determined by chromatographic methods. Pseudomonas sp. strain ISRH6 had strong ability to degrade the highly toxic AFB1, of 75.8% by culture supernatant compared to 64.5% and 63.2% by cell extracts and viable cells, respectively. Moreover, the results implied that the activity of aflatoxin B1 detoxification was mainly noticed in the cell supernatant of ISRH6 rather than cell extract and viable cells culture. The cell supernatant was characterized by considerable activity at wide range of temperature and pH.AFB1 degraded products were identified by GCMS, the compound BETA-MONOSTEARINE with retention time 38.844 of molecular weight 358.56 Da is the major degradative compound with good biological properties. The morphological, biochemical and 16S rRNA sequencing ofISRH6 revealed that belongs to Pseudomonas toyotomiensis and nucleotide sequence was provided with a GenBank accession number MG957214. Biosafety results indicated that AFB1 detoxification is an enzymatic and could have promising potential in industrial applications. First time we reported Pseudomonas toyotomiensis in the degradation of AFB1 by this research work.

Key words: Biodegradation, Aflatoxin B1, HPLC, GCMS, Pseudomonas toyotomiensis.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Manjunatha AS (2021). Detoxification of mycotoxin-aflatoxin B1 by soil bacterium Pseudomonas toyotomiensis . Acad. J. Microbiol. Res. 9(3): 055-068.

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