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Research Article

Academia Journal of Microbiology Research 9(1): 007-012, January 2020
DOI: 10.15413/ajmr.2020.1102
ISSN 2315-7771
2020 Academia Publishing


Abstract


Biochemical characterization and antibiotics sensitivity of bacteria associated with black rot of orange (Citrus sinensis) fruitin Bangladesh.
 

Accepted 27th December, 2020


Md.Faruk Hasan, Md. Asadul Islam and Biswanath Sikdar*

Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh
 

Citrus siensis is a delectable, juicy and prized fruit in Bangladesh, containing great nutritional impact. The present study was conducted for isolation and detection of the phyto-pathogen responsible for bacterial black rot disease of orange as well as evaluation of its antibioticsensitivity. The pathogen of the disease was isolated from infected fruits of orange and cultured on Luria-Bertani (LB) growth medium. Gram stainingand different biochemical testwereexposed for morphological characterization. Antibiotic sensitivity was assessed by disk diffusion method. The causal agents of the disease weregram negative, rod shaped, motile bacteria. In biochemical tests, Kligler iron agar (KIA), Simmon’s citrate tests were positive for bacterial sample 1 (BS1) and SIM (Sulphide-Indole-Motility) medium, MacConkey agar, catalase (H2O2), potassium hydroxide (KOH) tests were negative. Moreover, SIM medium, KIA, MacConkey agar tests were positive for bacterial sample 2 (BS2) and Simmon’s citrate, catalase, potassium hydroxide tests were negative. In antibiotic sensitivity’s assay, cefixime showed highest 28.00.7 mm diameter of inhibition zone against the isolated bacterial strains. The present research work may be helpful for molecular detection of citrus black rot diseases causing pathogens and their biological control techniques in near future.

Keywords: Citrus siensis, black rot, bacterial pathogen, identification, antibiotic sensitivity.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Hasan MdF, Islam MdA, Sikdar B (2021). Biochemical characterization and antibiotics sensitivity of bacteria associated with black rot of orange (Citrus sinensis) fruitin Bangladesh. Acad. J. Microbiol. Res. 9(1): 007-012.

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