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Research Article

Academia Journal of Scientific Research 6(4): 134-139, April 2018
DOI: 10.15413/ajsr.2018.0201
ISSN: 2315-7712
2018 Academia Publishing 

Abstract

 

 

Identification of methyl 4-hydroxybenzoate as a natural anti-Helicobacter pylori agent from the culture broth of Bacillus subtilis A81.
 

Accepted 13th February, 2018

 
Yuji Aiba1,2, Tomoshige Hiratsuka3, Toshio Nishizuka1, Hiroyuki Koshino3, Minoru Yoshida3 and Yasuhiko Komatsu1*

1Development Research Depratment, Snowden Co., Ltd., Tokyo 101-0032, Japan.
2Department of Psychiatry, Tokai University School of Medicine, Kanagawa 259-1193, Japan.
3Center for Sustainable Resource Science, RIKEN, Saitama 351-0198, Japan.

Novel bacilli producing anti-Helicobacter pylori compounds were screened from natural sources, and a new strain, Bacillus subtilis A81, was found to have strong anti-Helicobacter pylori activity. To determine the molecular structure of the anti-Helicobacter pylori compound(s) in Bacillus subtilis A81 cultures, we fractionated the culture supernatant containing activities with solvent extraction and silica gel chromatography. By analyzing with 1H and 13C nuclear magnetic resonance analyses, one compound was identified to be methyl 4-hydroxybenzoate (methylparaben). Helicobacter pylori was found to be relatively more susceptible (10-fold or more) to methylparaben than other bacteria, Staphylococcus aureus and Escherichia coli. To our knowledge, this might be the first report describing methylparaben as a natural anti-Helicobacter pylori product derived from B. subtilis.

Key words: anti-Helicobacter pylori activity, Bacillus subtilis, methyl 4-hyroxybenzoate, methylparaben, nuclear magnetic resonance, silica gel column chromatography.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Aiba Y, Hiratsuka T, Nishizuka T, Koshino H, Yoshida M, Komatsu Y (2018). Identification of methyl 4-hydroxybenzoate as a natural anti-Helicobacter pylori agent from the culture broth of Bacillus subtilis A81. Acad. J. Sci. Res. 6(4): 134-139

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