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Research Article

Academia Journal of Scientific Research 7(7): 413-418, July 2019
DOI: 10.15413/ajsr.2019.0204
ISSN: 2315-7712
©2019 Academia Publishing 

Abstract

 


Investigation of factors affecting the presence of
Lactobacillus spp. used in identification of vaginal secretion
 

 

Accepted 31st July, 2019

 
Eren Akgündüz1, Kenan Kaya2*, Ayşe Serin3, Ayça Ulubay3, Hüsniye Canan3 and Behnan Alper3

1Ministry of Justice, Council of Forensic Medicine, Kastamonu, Turkey.
2Ministry of Justice, Council of Forensic Medicine, Adana, Turkey.
3Department of Forensic Medicine, Cukurova University School of Medicine, Adana, Turkey.

 

The detection and identification of human body fluids at a crime scene is necessary in forensic anlysis, being a source for investigation and contributing to case evidence as regards the occurrence of an event. In addition to serological tests, methods based on human RNA analysis were developed for identification of body fluids and stains. The use of bacteria for the identification of body fluids was also being investigated and studies showed promising use of vaginal specific bacteria as a means of identifying vaginal secretions. In this study, the presence of the various Lactobacillus spp. including Lactobacillus gasseri, Lactobacillus crispatus and Lactobacillus jensenii were investigated in the vaginal swab samples taken from ninety-one (91) women cases with vaginal infections and postmenopausal. RNA isolation, cDNA synthesis and PCR were done respectively to 16S-23S rRNA intergenic spacer regions of the Lactobacillus spp. investigated. PCR products were run in capillary electrophoresis. It is believed that the negative results obtained cannot be used to exclude the presence of vaginal secretion as a result of undetected Lactobacillus spp in 12.9% of infection diagnosed samples and 46.6% of postmenopausal period cases. However, positive results may be valuable especially because no serological methods have been found in identification of vaginal secretion until now. In describing vaginal secretion, it is advisable to perform co-analysis of all three Lactobacillus species, and the presentation of any two Lactobacillus strains may support the possibility that the stain or biological material can be vaginal.

Key words: Sexual assault, Lactobacillus, RNA, vaginal secretion, body fluid identification.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Akgündüz E, Kaya K, Serin A, Ulubay A, Canan H, Alper B (2019). Investigation of factors affecting the presence of Lactobacillus spp. used in identification of vaginal secretion. Acad. J. Sci. Res. 7(7): 413-418.

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