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Research Article

Academia Journal of Scientific Research 7(10): 567-573, October 2019
DOI: 10.15413/ajsr.2019.0141
ISSN: 2315-7712
2019 Academia Publishing 

Abstract

 


Comparative analysis of Blood clot, Plasma rich in growth factors and Platelet rich fibrin resistance to bacteria induced fibrinolysis
 

Accepted 30th April 2019

 
Tomas Puidokas1, Mantas Kubilius1, Donatas Nomeika1, Gintaras Janužis2 and Erika Skrodenienė3

1Lithuanian University of Health Sciences, Department of Oral and Maxillofacial surgery, LT-46383, Kaunas, Lithuania.
2Lithuanian University of Health Sciences, Department of Laboratory Medicine, LT-46383, Kaunas, Lithuania.
 

The aim of this study was to evaluate the resistance of blood clot, Plasma Rich in Growth Factors (PRGF) and Platelet Rich Fibrin (PRF) to fibrinolysis induced by five different microbes and to assess the activity levels of these micro-organisms. Blood from forty-five (45) human volunteers was used to prepare four mediums: blood clot medium as control group, PRF and PRGF 1st fraction (PRGF I) and PRGF 2nd fraction (PRGF II) as study groups. Additionally, collected blood was used for blood plasma preparation on which evaluation of initial value of D-dimer concentration was performed. A solution of five different microbes (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumonia, Bacillus cereus and Candida albicans) was adjusted to 0.5 McFarland (1 108 CFU/ml) and then diluted to 0.25 McFarland (1 104 CFU/ml). The D-dimer concentration was evaluated after one and three hours of bacteria exposure. The resistance to fibrinolysis was not statistically distinguished between all medium groups at any time. S. pneumoniae was statistically active in PRF after three hours, while C. albicans was statistically active in PRGF II after one hour, in PRF between the first and third hour and after three hours. S. aureus and B. cereus were statistically active in PRGF II after three hours. S. pyogenes was statistically active after one hour between the first and third hour and after the third hour in all groups. S. pyogenes was the most active bacterium. Different blood formulations were not distinguishable based on resistance to bacteria induced fibrinolysis. Low fibrinolytic properties of the found major microbes defines the factor that bacteria-induced fibrinolysis is one of the leading causes of absence of a clot in a post-extraction socket as clinically insignificant. The initial absence of a clot or its mechanical elimination during formation or the healing period are major causes of dry-socket.

Key words: Platelet concentrate, fibrinolysis, dry-socket, D-dimer, bacteria.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:

Puidokas T, Mantas K, Donatas N, Gintaras J, Erika S (2019). Comparative analysis of Blood clot, Plasma rich in growth factors and Platelet rich fibrin resistance to bacteria induced fibrinolysis. Acad. J. Sci. Res. 7(10): 567-573.

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