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Research Article

Academia Journal of Scientific Research 7(10): 605-610, October 2019
DOI: 10.15413/ajsr.2019.0157
ISSN: 2315-7712
2019 Academia Publishing 

Abstract

 


Overexpression of ZNF797 (SALL4) gene in gastric cancer tissue, gastric cancer stem cell and MKN-45 cell line
 

Accepted 29th July 2019

 
Nilufar Shamlu1, Amir Nejad-Moghddam2, Fariba Ghasemvand3, Asal Safi Samghabadi3 and Saeid Rahmanzadeh3*

1Department of Biology and Microbiology Islamic Azad University of Parand, Tehran, Iran
2Marine Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3Enzyme Technology Lab., Genetics and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran.

 

Gastric cancer (GC) is one the most common types of cancer and leading causes of mortality worldwide. Recent studies have shown that ZNF797 plays an important role in the development and progression of different cancers. In the present study, ZNF797 expression was examined in different grades of primary human gastric cancer, gastric cancer stem cell (GCS) and MKN-45 cell line cell line. Gastric cancer tissue samples were collected from 37 patients with gastric cancer. Furthermore, MKN-45 cell line, GCS and 10 normal gastric tissue samples were prepared. Expression of ZNF797 at mRNA and protein levels was considered using Real-time PCR, flow cytometry, immunohistochemistry and western blot methods. Expression of ZNF797 was significantly increased in 46% of patients (3.3512.94; p<0.05) with gastric cancer. We also observed a significant increase in expression of ZNF797 in GCS (4.310.04; P<0.005). The mRNA expression level of ZNF797 in MKN-45 gastric cancer cell line was 2.810.07. Furthermore, ZNF797 expression was significantly correlated with tumor grade (p<0.01) and disease stage (p <0.01). ZNF797 may be involved in GC development and progression and can be considered as a prognosis factor for the diagnosis of this cancer, especially at the early stages. The oncogenic role of ZNF797 indicates that it may be a potential target for cancer therapy.

Key words: ZNF797, Cancer stem cell, MKN-45, expressional analysis, real-time PCR.

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:

Shamlu N, Amir N, Fariba G, Asal SS, Saeid R (2019). Overexpression of ZNF797 (SALL4) gene in gastric cancer tissue, gastric cancer stem cell and MKN-45 cell line. Acad. J. Sci. Res. 7(10): 605-610.

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