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Research Article

Academia Journal of Scientific Research Research 8(4): 123-131, April, 2020
DOI: 10.15413/ajsr.2020.0104
ISSN: 2315-7712
2020 Academia Publishing 

Abstract


Screening and characterization of lipase-producing, wax-degrading bacteria isolated from cotton mealybugs
 

Accepted 14th February 2020

Nagarathinam Arunkumar1,2, Jainullabudeen Gulsar Banu1, Nagarajan Gopalakrishnan1 and Arkalgud Hiriyannaiah Prakash1

1ICAR - Central Institute for Cotton Research, (CICR), Regional station, Coimbatore, Tamil Nadu - 641003, India.
2Department of Microbiology, School of Life Sciences, Central University of Tamil Nadu, Thiruvarur - 610 005, India.
 

 

Lipases (EC 3.1.1.3) are triacylglycerol acylhydrolases that hydrolyze triglycerides to glycerol and fatty acids. The numerous applications of lipases in chemical, food, pharmaceutical, biotechnological, detergents and surfactants, and agricultural industries and in waste treatment plants proved their importance. In this study, 23 wax-degrading bacterial strains isolated from four cotton mealybug species were screened for lipase production. On the basis of the 16S rRNA gene sequences, lipase-positive strains were classified into six genera, namely, Pseudoxanthomonas, Acinetobacter, Klebsiella, Providencia, Enterobacter, and Serratia. Acinetobacter lwoffii PSAD2 and A. beijerinckii PSAD7 isolates showed the maximum lipase production of 2.3 and 1.7 U/mL, respectively in the quantification process. The analysis of fatty acid methyl esters showed that A. lwoffii PSAD2 cells produced monounsaturated and hydroxyl fatty acids, whereas A. beijerinckii PSAD7 produced iso- and anteiso-methyl branched fatty acids. In addition, the growth curve analysis confirmed that lipase production is associated with the growth phase of the isolates. The results of this study will be helpful in developing lipase-based microbial insecticides for the management of waxy cuticle-protected insect pests.

Key words: Lipase, wax, bacteria, mealybug, 16S rRNA, fatty acid methyl esters.
 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:

Arunkumar N, Banu JG, Gopalakrishnan N, Prakash AH (2020). Screening and characterization of lipase-producing, wax-degrading bacteria isolated from cotton mealybugs Acad. J. Sci. Res. 8(3): 123-1.

 

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