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Research Article

Journal of Biological Series 2(3): 082-085, July 2019
DOI: 10.15413/jbs.2019.0108
2019 Academia Publishing

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Platelet anti-aggregation activity of hydrolized and irradiated crude fucoidan from Brown Seaweed (Sargassum polycystum)
 

Accepted 7th  June, 2019

 

Kartiningsih1,2, Deni Rahmat2, Partomuan Simanjuntak3 and Syamsudin Abdillah4*

1Faculty of Pharmacy, Universitas Pancasila, Indonesia.
2Department of Pharmaceutical of Technology, Faculty of Pharmacy, Universitas Pancasila, Jagakarsa, South Jakarta 12640, Indonesia.
3Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong, Bogor, Indonesia.
4Department of Pharmacology, Faculty of Pharmacy, Universitas Pancasila, Indonesia.

 

The platelet anti-aggregation activity of hidrolized and irradiatiated crude fucoidan from brown sea weed (Sargassum polycystum) was investigated in this study. The crude fucoidan of the brown seaweed was obtained by maceration using 2% CaCl2 (w/v). The platelet anti-agregation activity test was carried out using the modified LTA method. This study aimed at examining platelet anti-aggregation activity of hydrolyzed and irradiated fucoidan. Thereafter, it was degraded by gamma irradiation in doses of 20, 40 and 60 kGy, respectively. Furthermore, hydrolysis with HCl (4, 6 and 8%) and cellulose enzyme (20, 40 and 60 l) were also used to degrade fucoidan. The platelet anti-aggregation activity test was carried out using the modified LTA method. The results showed that the lowest molecular weight of degraded fucoidan obtained by gamma irradiation of 20 kGy was 753.16 Da and resulted in activity percentage of platelet anti-aggregation of 75.44%. Molecular weight of fucoidan hydrolyzed by HCl 8% was 714.48 Da, while its platelet anti-agregation activity was 61.91%. In addition, the cellulase enzyme hydrolysis of 60 l produced degraded fucoidan with molecular weight of 714.67 and its platelet anti-agregation activity was 64.30%. It was concluded that the molecular weight reduction of fucoidan molecules by gamma irradiation demonstrated the highest platelet anti-aggregation activity.

Key words: Brown seaweed, crude fucoidan, HCl hydrolysis, cellulase enzyme, gamma irradiation, platelet anti-agregation.

 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this article as:
Kartiningsih, Rahmat D, Simanjuntak P, Abdillah S (2019). Platelet anti-aggregation activity of hydrolized and irradiated crude fucoidan from Brown Seaweed (Sargassum polycystum). J. Biol. Ser. 2(3): 082-085.

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